Basic Research - Cancer(구연) (NP-002) hsv2-miR-H9 and hsv1-miR-H18 in Formalin-Fixed Paraffin-Embedded (FFPE) are valuable diagnostic biomarkers for Prostate Cancer 충북대학교 의과대학 비뇨기과학교실 변영준, 박현미, 정필두, 서성필, 강호원, 김원태, 김용준, 윤석중, 이상철, 김원재 Background: Previously, we investigated the expression of two viral miRNAs (hsv2-miR-H9 and hsv1-miR-H18) in prostate cancer (PCa) from fresh tissues and urine. In this study, we reconfirmed two viral miRNAs as field-effect related biomarkers for PCa in FFPE (Formalin-Fixed Paraffin-Embedded) and could generate hypothesis with these results. Materials and Methods: In total, FFPE tissue samples from 67 PCa patients, 100 noncancerous surrounding tissues, 100 benign prostate hyperplasia (BPH) were analyzed by real-time polymerase chain reaction (RT-PCR) in this study. Results: The expression levels of two viral miRNAs were significantly higher in FFPE of PCa than in BPH (P<0.001). Remarkably, the expression levels of two viral miRNAs in noncancerous surrounding tissues also were higher than in BPH controls (P<0.005). In case of PSA levels below 10ng/mL, hsv1-miR-H18 could discriminate PCa, noncancerous surrounding and BPH from each other (P<0.001). Conclusion: Our finding suggests that hsv2-miR-H9 and hsv1-miR-H18 may help for decreasing unnecessary biopsy with increasing positive detection for PCa as valuable diagnostic biomarkers. keywords : viral miRNA, miRNA, Formalin-fixed paraffin-embedded, Prostate cancer