Basic Research - Cancer(구연) (NP-004) Down expression of HLA-DRA gene：an independent prognostic predictor tends to well prognosis in non-muscle invasive bladder cancer 충북대학교 의과대학 비뇨기과학교실 박현미, 김예환, 정필두, 이희윤, 서성필, 강호원, 김원태, 김용준, 윤석중, 이상철, 김원재 Demand for identification of prognostic immune biomarker has increased given the emerging role of immune biomarkers in tumorigenesis or anticancer immunotherapy. Prognostic significance of immune markers has been proved in various cancers, but that in bladder cancer (BC) has yet been evaluated. To clarify the role of the antigen-presenting molecule human leukocyte antigen (HLA)-DRA expression in BC development, the present study investigated HLA-DRA mRNA expression in non-muscle invasive bladder cancer (NMIBC) and muscle invasive bladder cancer (MIBC) tissue samples using real-time polymerase chain reaction assay. A similar expression levels of HLA-DRA were determined in MIBC and high grade NMIBC patients, those were significantly higher than that in low grade NMIBC patients (P<0.05, respectively) (Fig. 1); in addition, HLA-DRA mRNA expression increased in NMIBC patients who progressed to MIBC later. The Kaplan-Meier and cox regression analysis showed lower expressed HLA-DRA in NMIBC patients was an independent predictor of better prognosis (Fig. 2), associated with a 3.513 times increase (95% CI: 1.190-10.373) in progression free survival. In conclusion, higher expression of HLA-DRA in NMIBC patients has implications for patient stratification strategies as well as BC tumor immunology. keywords : non-muscle invasive bladder cancer, immune-related biomarker, HLA-DRA