Basic Research - Neurourology & LUTS/BPH & Others(구연) MP Session Ⅱ (MP-011) Geumkang Hall (Avenue 2F) 11월 28일(수) 17:00-18:00 Cyclophosphamide에 의해 유발된 방광염을 동반한 rat 방광에서 Aquaporin 2와 Caveolin 2의 발현 전남대학교 의과대학 비뇨기과학교실 임도경, 김재현, 김태희,은성종, 오주용, 오정훈, 손수민, 정세현, 유성현, 김명수, 정호석, 유호송, 오경진, 김선옥, 정승일, 강택원, 권동득, 박광성 Purpose: The purposes of this study were to investigate the effect of cyclophosphamide (CYP)-induced inflammatory cystitis on aquaporin 2 (AQP 2) and caveolin 2 (CAV 2) in rat urinary bladder and to determine the role of these molecules in the detrusor overactivity that occurs in inflammatory change in rat urinary bladder. Methods: Female Sprague-Dawley rats were divided into control (n=30) and experimental (n=30) groups. Cystitis in experimental group was induced by intraperitoneal injection of CYP (200mg/kg). The control group underwent an intraperitoneal saline injection. After 2 days, urodynamic studies were done to measure the contraction interval and contraction pressure. The expression and cellular localization of AQP 2 and CAV 2 were determined by Western blot and immunofluorescent study in rat urinary bladder. Results: In cystometrograms, the contraction interval (min) was significantly lower in the CYP-induced cystitis rats (6.3 ± 0.5) than in the control group (13.8±3.2)(p<0.05). Conversely, the average contraction pressure (mmHg) was significantly higher in the CYP-induced cystitis rats (14.8±2.5) than in the control group (11.0±1.2)(p<0.05)(Fig. 1). AQP 2 was expressed in the cytoplasm of the detrusor muscle cell, whereas CAV 2 was expressed in the cell membrane of the detrusor smooth muscle cell. The protein expression of AQP 2 and CAV 2 was significantly decreased in the CYP -induced cystitis rats (p<0.05). Conclusions: Inflammatory change of urinary bladder maybe causes a significant change in the expression of AQP 2 and CAV 2. These findings suggest that AQP 2 and CAV 2 might have a functional role in the detrusor overactivity related with cystitis in rat urinary bladder. keywords : aquaporin 2, caveolin 2, cystitis