Basic Research - Cancer(구연) Oral Session 3 / Basic Research - Cancer (Ⅱ) (O-030) Rm.201 10월 30일(수) 14:00-15:00 TRPM7 : 방광암 치료를 위한 새로운 치료 표적 칠곡경북대학교병원, ¹경북대학교병원 하윤석, 정재욱, 최석환¹, 이준녕, 김범수¹, 김현태, 김태환, 유은상¹, 권태균 Purpose: Transient receptor potential melastatin 7 (TRPM7) functions as a Mg2+/Ca2+- permeable channel has been linked with a kinase domain and regulates various physical processes and diseases. As the function of TRPM7 in human bladder cancer (Bca) has not been fully understood, we aim to investigate the possible effects and potential mechanisms of TRPM7 on cell proliferation and metastasis in Bca cells. Its therapeutic potential with in vivo animal models was also studied. Methods: The human Bca cell lines T24 and UMUC3 were chosen for this study. The molecular mechanisms of TRPM7 action were studied using Western blot analysis and small interfering RNA (siRNA)-based knockdown. We also described the anticancer effects and mechanism of action of Carvacrol, a nonselective TRPM7 inhibitor, with in vitro and in vivo data on Bca. Results: siRNA-induced silencing of TRPM7 notably inhibited BCa cell proliferation, migration and invasion, as well as suppress the phosphorylation levels of Src, Akt and JNK at the protein level. Additionally, the treatment of cells with Src, Akt and JNK inhibitors clearly limited the proliferation, migration and invasion of T24 and UMUC3 cells. Systemic administration of Carvacrol in mice bearing bladder xenograft tumors revealed delayed tumor growth. Conclusions: Taken together, our results argue that TRPM7 plays an important role in proliferation, migration and invasion of T24 and UMUC3 Bca cells via the Src/Akt/JNK signaling pathway. Targeting these critical interactions with a nonselective TRPM7 inhibitor offers a novel strategy to therapeutically manage Bca. (2019R1H1A1079839) keywords : TRPM7, Bladder cancer, Treatment