Basic Research - Neurourology & LUTS/BPH & Others(구연) Oral Session6 / Basic Research - non-Cancer (I): Neurourology (O-062) Rm.203 10월 30일(수) 15:00-16:00 방광내 히알루론산 및 염화칼륨 혼합액 주입을 이용한 만성간질성방광염의 동물모델확립 대구 파티마병원 비뇨의학과¹ 대구 파티마병원 재활의학과² 대구 파티마병원 의학실험실³ 피츠버그대학교 의과대학 비뇨의학과교실⁴ 권준범¹, 박동휘², 이은주³, 조현정³, 장지애³, 한민수³, 이윤형¹, 김재수¹, 요시무라 나오키⁴ The pathophysiology of interstitial cystitis (IC) has not been clearly revealed, Currently lots of animal model has been used for the pathophysiology of IC, but most of them are fit for acute inflammation model not chronic model. So we tried to establish the chronic animal model of IC using intravesical hyaluronidase, which can degrade GAG layer, mixed with KCl. 12 female mice were divided into 3 groups (each N=4) : 1) Sham group, 2) 1 wk group from the last hyaluronidase, 3) 3 wks group from the last hyaluronidase. Both 1 wk and 3 wks group were treated with hyaluronidase once a week for 3 wks, and then pain assessment, CMG, histology, measurement of transcripts representing urothelial tight junction and inflammatory change using real time PCR were conducted after 1 week and 3 weeks from the last hyaluronidase. In pain assessment, 50% thresholds were decreased significantly in both 1 week and 3 weeks compared with sham group (Fig. 1). The results of CMG among three groups were represented in Table 1. In histology, mucosal thinning and denudation in urothelium was seen at both 1 wk and 3 wks. However these changes were not severe or extensive but regional. Inflammatory cells were infiltrated broadly but not severely, either. mRNA of ZO-1 was reduced at both 1 wk and 3 wks. IL-1β, IL-6, CCL2, and VEGFα were up-regulated at both 1 wk and 3 wks (Fig. 1). Our model still showed persistant tactile allodynia in pain assessment and chronic inflammation in pathology and RT-PCR after 3wks from damage. Therefore, thought to be adequate for chronic model of interstitial cystitis. keywords : Interstitial Cystitis, Pain, Chronic Model