Basic Research - Neurourology & LUTS/BPH & Others(구연) Oral Session6 / Basic Research - non-Cancer (I): Neurourology (O-061) Rm.203 10월 30일(수) 15:00-16:00 뇌, 비장, 췌장 및 신장을 비롯한 여러 장기의 생리학적 혈관 구조를 위한 진보된 조직 투명화 영상 기술 ¹경북대학교 의학전문대학원 비뇨기과학교실 ²경북대학교병원 비뇨기과학교실 ³칠곡경북대학교병원 비뇨기과학교실 ⁴경북대학교 의학전문대학원 해부학교실 하헌², 권순오², 이승윤³, 오정석², 김진우², 정재욱³, 하윤석¹·³, 이준녕¹·³, 김범수¹·², 김태환¹·³, 유은상¹·², 권태균¹·³, 오지원⁴, 최석환¹·² Introduction: Due to the obscuring effects of light scatter and technical limitation of current histochemistry, deep imaging into a tissue is problematic in last decade, there has been a great progress of tissue clearing methods that make large biological samples transparent and allow unprecedented three-dimensional views of variety of tissues. However, the three-dimensional view of vasculature systems of each different organ is largely unexplored. Here, we investigated the structure of vascular system in eyeball, brain, liver, spleen, pancreas and kidney using tissue clearing methods. Methods: For the blood vessel staining, we sacrificed the mice and attached the Lycopersicon Esculentum Lectin using systemic cardiac circulation onto each different organs. We took more than thousands of still shot pictures of vasculature system of each organs based on lectin binding images under light-sheet microscope, requiring three-dimensional reconstruction by Imaris 9.3 image analysis software. Results: Reconstructed images gave us the detailed topological information of interconnectional status of vasculature. (Figures) Conclusions: Tissue clearing methods with reconstructed three-dimensional images allows for quantification of the interconnected status of tissue vasculature as well as spatial insight for the physiological conditions. Furthermore, combined with immune-histochemistry antibody reaction within tissue clearing methods, it can give us topological information of specific cellular populations in the context of vascular system. keywords : Tissue clearing method, vasculature system