Basic Research - Cancer(구연) Oral Session1 / Basic Research - Cancer (Ⅰ) (O-008) Rm.201 10월 30일(수) 13:00-14:00 Effects of patch combining human fibroblast-derived matrix on erectile function in a bilateral cavernous nerve injury rat model 가톨릭대학교 서울성모병원 신동호, 정애량, 문형우, 박용현, 김인걸, 이지열 Purpose: In prostate cancer patients, erectile dysfunction after prostatectomy is a major problem. Recently, an approach to tissue engineering has been tried on post-prostatectomy erectile dysfunction, spatio-temporally controlled interactions between cells, growth factors, and extracellular matrix(ECM) is an important factor in achieving normal structural integrity in nerve regeneration. In this study, we have attempted to produce a novel patch that physically combines human fibroblast-derived matrix(hFDM) and human mesenchymal stem cells(hMSC). Methods: Decellularized human fibroblast-derived matrix(hFDM) was prepared in tissue culture plate and PVA solution was injected. After the freeze-thaw process, PVA was stripped from TCP with hFDM, which was firmly combined to PVA. We investigated the efficacy of PVA/hFDM patch with hMSCs on the cavernous nerve in a BCNI rat model. Rats were randomly divided into five groups: Normal group, BCNI group, hMSC(5x105), PVA/hFDM patch+hMSC(1x105)group and PVA/hFDM patch+hMSC(5x105) group. Erectile function was assessed by measuring the intracavernous pressure(ICP)/mean arterial pressure(MAP) level, Penile and cavernous nerve tissue were retrieved for histologic and molecular analyses. Results: In vitro analysis, PVA/hFDM patch+hMSC shows significantly increased expression of Nestin, GFAP that indicated as a marker of neural development. In animal experiments, the ICP/MAP ratios in the patch group were significantly increased compared to those in the BCNI groups, patch group showed significantly increased smooth muscle/collagen ratio, nNOS contents, phospho-eNOS protein expression, and cGMP level, compared with the BCNI group. Conclusions: PVA/hFDM patch+hMSC could significantly improve erectile function and alleviate pathological changes in a BCNI rat model. PVA/hFDM patch+hMSC may be a potential agent for post-prostatectomy ED. keywords : Erectile dysfunction, stem cell