Basic Research - Neurourology & LUTS/BPH & Others(구연) Oral Session6 / Basic Research - non-Cancer (I): Neurourology (O-060) Rm.203 10월 30일(수) 15:00-16:00 The application of optogenetics in detrusor underactive mouse models 성균관의대 삼성서울병원 ¹의공학연구센터, ²비뇨의학교실 박은경¹, 김태경¹, 주재순¹, 임봉희², 이규성¹² Introduction Detrusor underactivity (DUA) is defined as a contraction of reduced strength and/or duration resulting in prolonged or incomplete emptying of the bladder. The current lack of effective pharmacological and surgical therapy makes patients dependent on conservative management, such as scheduled voiding and self-catheterization. In this study, we aim to overcome the limitation of the existing treatments and suggest a novel treatment method using optogenetics based on preclinical test. Materials and methods In order to develop DUA animal model, the mice were induced diabetic by streptozotocin injection and selected through the metabolic cage study. The opsin-packaged adeno-associated virus (AAV9-ChR2-EGFP) was injected into the delicate region between the serosal and muscular layers of bladder. After three weeks virus injection, cystometry was performed with bladder catherization and decerebration. The optical stimulation (470nm, 26mW) was applied for 1 second at random moments. Four urodynamic parameters (baseline pressure (BP), maximum voiding pressure (MVP), inter-contraction interval (ICI), voiding volume (VV)) had been measured during spontaneous voiding (SV) and light-induced voiding (LIV). Results Analysis showed that when comparing SV and LIV respectively, there were no significant differences in the means for BP and for MVP (p > 0.1). On the other hand, the test did show a statistically significant difference in the means for ICI with the ICI during SV being longer than the ICI during LIV (p < 0.05). Lastly, there was also a statistically significant difference in the means of VV with that of SV being higher than that of LIV (p < 0.05). Conclusion This study presents that bladder contraction can be induced in ChR2-expressing murine bladders of animal models, and temporally and spatially controlled in response to blue light illumination. This is an important step towards improved patient care for people suffering from DUA. keywords : Optogenetics, Detrusor Underactivity, Adeno-associated virus