Basic Research - Cancer(구연) Oral Session1 / Basic Research - Cancer (Ⅰ) (O-006) Rm.201 10월 30일(수) 13:00-14:00 Urinary mRNA using droplet digital PCR for prostate cancer screening in a Korean population 충북대학교 의과대학 비뇨의학교실 강호원, 변영준, 박현미, 정필두, 이희윤, 서성필, 김원태, 김용준, 윤석중, 이상철, 김원재 Objective: To evaluate the clinical performance of urinary mRNA expression using droplet digital PCR (ddPCR), which could be a useful method for the quantifying low concentration of urinary mRNA as a noninvasive urine-based marker for prostate cancer (PCa) diagnosis. Materials and methods: Gene expression microarray (HT-12, illumina) was performed to find differentially expressed genes between PCa tissue and benign prostatic hyperplasia tissues. To quantify selected genes in tissue and urine, ddPCR (QX200, Biorad) was carried out. The urinary molecular PCa risk score (UMPCaRS) was calculated as the ratio of candidate genes with sum of 3 up-regulated genes as the numerator and sum of 3 down-regulated gene as the denominator. The diagnostic value of UMPCaRS was validated in screening set (10 PCa and 10 BPH) and validation set (49 PCa and 36 BPH). Results: Using gene expression microarray, 3 over-expressed genes (PDLIM5, GDF-15, THBS4) and 3 down-expressed genes (UPK1A, SSTR3, NPFFR2) were selected for digital PCR. In screening set, mRNA expression of selected genes in tissue and urine were showed similar patterns with array data. The diagnostic accuracy of UMPCaRS was superior to that of PSA. UMPCaRS had an area under the curve (AUC) of 0.895, a sensitivity of 86.1%, a specificity of 83.7% in validation set. The AUC was 0.879 for the UMPCaRS model versus 0.555 for PSA for patients with PSA levels between 3 and 10 ng/m (P= 0.012). Conclusions: Our data demonstrated that UMPCaRS could serve as a noninvasive urine-based marker for prostate cancer (PCa) diagnosis. keywords : Prostate carcinoma, Urine, Diagnosis,