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Basic Research - Neurourology & LUTS/BPH & Others(구연)
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Oral Session6 / Basic Research - non-Cancer (I): Neurourology (O-058)
Rm.203
10월 30일(수) 15:00-16:00
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The role of Galectin-3 in bladder remodeling in rat with spinal cord injury : candidate biomarker and therapeutic target |
| 연세대학교 의과대학 비뇨의학교실 |
| 성형경, 이용승, 한상원, 김상운 |
Purpose
Galectin-3 belongs to the galectin family of mammalian lectins and is characterized by a carbohydrate recognition domain that has affinity for β-galactosides. Galectin-3 mediates cell–cell and cell–matrix interactions and there is mounting evidence demonstrating its key role in inflammatory and fibrotic processes. In this study, we investigate the role of Galectin-3 in bladder fibrosis and whether it is a relevant therapeutic target in bladder remodeling by assessing galectin-3 expression in urine and bladder with rat model of spinal cord injury (SCI).
Methods
The spinal cord was transected at the level of T8-9 in female Sprague-Dawley rats (200-250g), which were divided into three groups; A: Sham, B: SCI, C: SCI with treatment (galectin-3 inhibitor, modified citrus pectin, MCP) groups, respectively. MCP was orally administered (2ml/kg/d) after spinal cord transection. Cystometrogram was conducted 1 week and 4 weeks after SCI in each group. We evaluated urodynamic parameters and bladder tissue remodeling factors.
Results
Non-voiding contractions (NVCs) during the storage phase of cystometrograms tended to be increased in all two SCI groups (B and C) with a significant reduction in treatment group (C) versus B. Bladder compliance was improved, and intercontraction intervals were increased in group C compared with group B. In two groups with SCI (B and C), the expression of galectin-3 and other fibrosis markers (a-SMA, collagen III) was increased compared to group in A while decreased expression of all fibrosis markers was noticed in group C (MCP treatment). In group C, the reduction of AKT signaling pathway was demonstrated with treatment of MPC for 4 weeks. The urine galectin-3 level was increased in SCI groups with a reduction of group C versus group B by ELISA.
Conclusion
The expression of galectin-3 was increased in SCI groups with other fibrosis markers of bladder. Galectin-3 inhibitor comparably improve bladder capacity in rat, by downregulating galectin-3 and reducing bladder fibrosis.
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keywords : Spinal cord injury, galectin-3, fibrosis |
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