Basic Research - Neurourology & LUTS/BPH & Others(구연) Oral Session6 / Basic Research - non-Cancer (I): Neurourology (O-057) Rm.203 10월 30일(수) 15:00-16:00 노화 쥐 방광에서 카베올린(Caveolin) 1,2의 발현 감소 : 노령 인구에서 방광 기능저하의 표지자 전남대병원 비뇨의학과. 송재익, 이호연, 김재현, 임도경, 김태희, 은성종, 오주용, 유성현, 김명수, 정호석, 유호송, 오경진, 김선옥, 정승일, 강택원, 권동득, 박광성 Purpose: Caveolins (CAVs) are specialized regions of the cell membrane that modulate signal transduction and alterations in these structures affect bladder smooth muscle contraction. Since bladder dysfunctions are common in the elderly, we evaluated the effect of aging on the morphology of caveolin (CAV1-2) protein expression in bladder tissue. Methods: Female Sprague-Dawley rats were divided into young (10 weeks, n=30) and aging (20 months, n=30) groups. The urodynamic studies were done to measure the contraction interval and contraction pressure. The expression and cellular localization of CAV1-2 and Collagen 1A2 (COL1A2) were determined by Western blot and immunofluorescent study between the groups in the urinary bladder. Results: In cystometrograms, the contraction interval (min) was significantly lower in the aging rats (3.7 ± 0.5) than in the young group (6.2 ± 0.8)(p<0.05). The average contraction pressure (mmHg) was significantly higher in the aging rats (8.4 ± 0.6) than in the young group (13.2 ± 1.3)(p<0.05). CAV1-2 and COL1A2 were expressed in the capillaries, arterioles, venules and smooth muscles. The CAV1-2 and COL1A2 protein expression were significantly decreased in the aging rat compared with young control (p<0.05). Conclusions: There was significant decrease in the expression of CAV1-2 and COL1A2 in the aging rat urinary bladder. Since caveolae are protected membrane regions that regulate signal transduction, age-related alterations in CAV protein expression could alter bladder smooth muscle contractility resulting in bladder dysfunctions of the elderly. keywords : Aging, Caveolin, Rat