Incontinence & Female Urology & Neurourology(구연) Oral Session2 / Incontinence & female urology (O-015) Rm.203 10월 30일(수) 13:00-14:00 Overexpression of SDF-1 Improves Neurogenic Bladder by Inducing Stem Cells Homing in a Pelvic Nerve Crush Injury Rat Model 가톨릭대학교 의과대학 비뇨기과학교실 성재우, 신동호, 양종협, 조신제, 문형우, 이규원, 박용현, 조혁진, 하유신, 홍성후, 이지열, 김세웅, 배웅진 BACKGROUND: There is still a lack of sufficient research on the mechanism behind neurogenic bladder (NB) treatment. OBJECTIVE: To explore the effects of over-expressed stromal cells-derived factor-1 (SDF-1) microenvironment produced by engineered immortalized mesenchymal stem cells (imMSCs) on the NB and investigate underlying mechanism. Materials & Methods: Bone marrow mesenchymal stem cells (BM-MSCs) were transfected to immortalized up-regulated SDF-1 engineered BM-MSCs (imMSCs/eSDF-1➕) or immortalized normal SDF-1 engineered BM-MSCs (imMSCs/eSDF-1➖). Male rats were divided into four groups: sham, NB, NB+imMSCs/eSDF-1➕, and NB+imMSCs/eSDF-1➖. NB rats were induced by major pelvic ganglion (MPG) injury. INTERVENTION: One weeks after NB model established, sham and NB rats were performed to a vehicle injection in the MPG. Rats in other two groups received engineered imMSCs injection. OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: After a four-week treatment, bladder function was assessed by cystometry and voiding pattern analysis. The MPG and bladder tissues were evaluated through immunostaining and Western blotting. RESULTS AND LIMITATIONS: The imMSCs/eSDF-1➕ over-expressed SDF-1 and improved NB evidently. SDF-1 induced MSCs homing to the injured MPG. The imMSCs/eSDF-1➕accelerated regeneration of bladder wall in NB rats by activating PI3K/AKT/mTOR pathway and recovered injured nerve more effectively via stimulating MAPK Pathway. Over-expressed SDF-1 increased VEGF and bFGF, meanwhile it decreased apoptosis in the MPG. Our results are limited to the MSCs treatment was performed at an early stage of prostatitis development. CONCLUSIONS: Our finding proved over-expressed SDF-1 efficiently induced more MSCs homing to the injured tissue, which improved the NB via accelerating restoration of injured nerve in a rat model. keywords : In an experimental setting, BM-MSCs treatment improved NB effectively