Incontinence & Female Urology & Neurourology(구연) Oral Session2 / Incontinence & female urology (O-014) Rm.203 10월 30일(수) 13:00-14:00 방광 내 골반신경 자극 후 Auqaporin2(AQP2) 및 Vasopressin v1a (AVP-v1a)의 발현 및 막전좌 전남대학교병원 비뇨의학과 김태희, 김재현, 김선옥, 송승희, 강택원, 권동득, 박광성, 송재익 Introduction: Aquaporins (AQPs) are membrane proteins that facilitate water movement across biological membranes. The purposes of this study were to investigate the localization and functional roles of AQP2 and vasopressin v1a (AVP-v1a) receptor in the urinary bladder. Methods: Female Sprague-Dawley rats (230–240 g, N = 20) were anesthetized. The pelvic nerve was stimulated for 60 seconds (10 V, 16 Hz, 0.8 ms), and the animals were sacrificed immediately after nerve stimulation. The expression and cellular localization AQP2 and AVP-v1a of were determined by Western blot and immunohistochemistry of the urinary bladder. The intracellular membrane and plasma membrane fractions of the proteins in bladder tissues were studied by immunoblot analysis with the differential centrifugation. Results: Immunolabeling showed that AQP2 and AVP-v1a were mainly expressed in the cytoplasm of the urotheliym of the urinary bladder. AQP2 and AVP-v1a were found to be present primarily in the cytosolic fraction of untreated tissues. The translocation of AQP2 and AVP-v1a from the cytosolic compartment to the membrane compartment was observed after nerve stimulation. Conclusions: These results showed a distinct localization of AQP2 and AVP-v1a in the rat urinary bladder. Pelvic nerve stimulation modulated immediate translocation of AQP2 and AVP-v1a. These results imply that AQP2 and AVP-v1a may play an important role in bladder activity by nerve stimulation of the urinary bladder. keywords : AQP2, AVP, bladder