Basic Research - Cancer(구연) Oral Session1 / Basic Research - Cancer (Ⅰ) (O-002) Rm.201 10월 30일(수) 13:00-14:00 비뇨기암 환자에서 소변의 세균유래 소포체를 이용한 메타제놈 분석 ¹이화여자대학교 의과대학 비뇨의학과 교실 ²이화여자대학교 의과학연구소 이은주¹², 송완¹, 윤하나¹, 정우식¹, 심봉석¹, 이동현¹, 김광현¹ Purpose: Bacteria derived extracellular vesicles (EV) contains bacterial DNA fragments and EV can be isolated from various body fluid. In this study, we aimed to evaluated bacterial derived EV in urine from patients with urologic malignancies. Methods: The study was conducted with urine DNA of 54 cancer patients and 17 healthy individuals. We performed a 16S rRNA gene sequencing using Illumina MiSeq to analyze the urine microbiota. Assignment of taxonomy was performed on using pipeline of QIIME taxonomy classification methods. Generalized linear models implemented in multivariate association with linear models (MaAsLin) packages of RStudio were used for quantitative and qualitative analysis. Results: Of the 71 samples, we excluded samples (8 samples of the cancer group and 9 samples of the healthy control group) that showed too low sequencing reads (less than 3500 reads). A total of 54 samples were analyzed, including 46 from cancer group (including 21 bladder, 16 prostate, and 9 renal cancer subjects) and 8 healthy controls. There was no significant difference in age between cancer and healthy control group. Prostate cancer groups (n=16) had a distinct bacterial community compared to healthy control group (n=8) and other cancer groups (n=30). In the urine exosome derived microbiota, the Microbial diversity were significantly decreased bacterial evenness (pielou_e, Control vs Prostate cancer; p=0.0275, Bladder vs Prostate cancer; p=0.008, Prostate vs Renal cancer; p=0.0127, respectively) and diversity (Shannon index, Control vs Prostate cancer; p=0.028, Prostate vs Renal cancer; p= 0.031, respectively) in Prostate cancer group. Also, enrichment of some bacterial genera (e.g., Peptoniphilus, Pseudomonas, Propionibacterium q < 0.25) in Prostate cancer groups when compared to healthy control group and other cancer groups. Conclusion: The urine exosome derived microbiota profiles showed different patterns between prostate cancer group and healthy control group or other cancer groups (bladder cancer and renal cancer). Detected microbiome alterations suggest that microbiome may be implicated in physiologic role in prostate cancer. keywords : microbiota, urologic malignancy, extracellular vesicle, urine