Basic Research - Cancer(구연) Oral Session1 / Basic Research - Cancer (Ⅰ) (O-001) Rm.201 10월 30일(수) 13:00-14:00 DRG2 level in prostate cancer cell line impacts docetaxel-induced apoptosis. 울산대하교 의과대학 1이비인후과, 2해부병리과, 3비뇨의학과 김송희, 이원혁, 김영민, 문경현, 박성찬 Introduction: DRG2 is a member of the DRG subfamily in the GTP-binding protein superfamily. This study was aimed to investigate whether DRG2 has a role in cell-cycle regulation of prostate cancer cell line. Materials and Methods: LNCap-LN3, PC3 and DU145 prostate cancer cell line were used to reveal change of cell cycle and expression of DRG2 after docetaxel treatment. The type of cell death induced by docetaxel was evaluated by Annexin V binding assay. Results: DRG2 expression was high in LNCap-LN3 and low in DU145. DRG2 expression decreased in proportion to the docetaxel concentration in all three cell lines. FACS analysis revealed docetaxel induced G2/M phase arrest in LNCap-LN3 and PC3 cell lines with relatively high expression of DRG2. In DU145 with low expression of DRG2, docetaxel induced apoptosis. After knock down of DRG2 in PC3 cell line, docetaxel induced apoptosis instead of G2/M phase arrest. Conclusions: DRG2 expression in prostate cancer cell line was reversely correlated with cancer aggressiveness. DRG2 had a major role in docetaxel-induced apoptosis of prostate cancer cell line. keywords : DRG2, Prostate cancer, docetaxel