Basic Research - Cancer(구연) International Session I (I-07) Rm. 202 10월 31일(목) 15:00-16:00 Preparation and analysis of Urine to detect androgen receptor by RT-PCR 부산대학병원 의생명과학 연구소¹ 부산대학교 의과대학 비뇨의학과교실² 후다 나즈물¹, 구자윤¹², 하홍구¹² Purpose: Detection of androgen receptor full length (AR-FL) and androgen receptor variant 7 (AR-V7) is promising as a clinically significant biomarker in prostate cancer. Promptly obtainable samples of liquid biopsies such as urine may hold different types of circulatory cells, biomarkers, protein, DNA, and RNA. Nowadays, non-invasive liquid biopsies have appeared as a substitute to tumor biopsy but existing methods to isolate and depict RNA molecules in urine for RT-PCR are limited. Here, we present the technique to extract RNAs from urine without the necessity of blood withdrawal and tissue samples. Materials and Methods: The present experiment narrates the methods for extracting RNA from urine that leads to detect androgen receptors by RT-PCR. Initially, the urine sediment such as cells, organelles, other cellular debris, and insoluble mineral crystals may be physically or functionally separated from the soluble urine fraction. Then RNA is extracted from urine that using different methods such as manual Trizol method, and two different types of commercial available kits. Different prostate cancer patients’ urine samples might show different level of AR detection but we have setup a standard protocol to get good quality as well as satisfactory amounts of RNA using less amount of urine samples that leads for further experiment like RT-PCR to detect androgen receptors and other biomarkers. Conclusions: The techniques described in this study is appropriate for large amount of RNAs in small amount of urine to detect androgen receptors for wide variety of health and disease analyses, especially prostate cancer. keywords : biomarker, urine, androgen receptor variant 7