Basic Research - Infertility & Sexual Dysfunction(구연) (E-023) 모틸리펌은 쥐에서 고정된 자세에의한 스트레스로 발생하는 고환기능장애를 감소시킨다 전북대학교병원, , 성균관의대 삼성서울병원, 한양대학교 약대,경성대학교 약대 케샵카르나¹, 신유섭¹, 최나영¹, 이성원², 김철영³, 김혜경⁴, 박종관¹ Objectives: Psychological stress appears to be one of the main causes to dysfunction of male reproductive health. MOTILIPERM extract is composed by three crude medicinal herbs [Morinda officinalis How (Rubiaceae) roots, Allium cepa L. (Liliaceae) outer scales and Cuscuta chinensis Lamark (convolvulaceae) seeds]. The present study aimed to investigate the possible mechanism for the effects of MOTILIPERM on testicular dysfunction induced by immobilization stress. Methods: Fifty male Sprague Dawley rats were equally divided into five groups (10 rats each): normal control group (CTR), a control group administered MOTILIPERM 200 mg/kg (M 200), a stress by immobilization-induced control group (S), and a stress by immobilization-induced group administered MOTILIPERM 100 mg/kg (S + M 100) and MOTILIPERM 200 mg/kg (S + M 200). Stressed rats (n = 30) were subjected to stress by immobilization for 6 h by keeping in perspex restraint cage and controls (n = 20) were maintained without disturbance. Rats were administrated 100 and 200 mg/kg MOTILIPERM once daily for 30 days 1 h prior to immobilization. Results: MOTILIPERM treatment improves testicular dysfunction by up-regulating (P 〈 0.05) sperm count, sperm motility; serum testosterone level; StAR protein level, Johnsen score, spermatogenic cell density of stressed rats. MOTILIPERM decreases oxidative stress by increasing (P 〈 0.05) testicular superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase; decreasing (P 〈 0.05) malondialdehyde (MDA) and reactive oxygen species/reactive nitrogen species (ROS/RNS) level. Further, MOTILIPERM down-regulated (P 〈 0.05) cleaved caspase 3, Bax; increased pro caspase-3, Bcl-2 and upregulate testicular germ cell proliferation in stressed rats. The serum luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels also significantly (P 〈 0.05) decreased after pretreatment of MOTILIPERM in stressed rats. Conclusions: Our results suggested that, MOTILIPERM ameliorate alteration in spermatogenesis under immobilization stress via oxidative stress and mitochondrial apoptosis pathway. keywords : Stress by immobilization, MOTILIPERM, testis, oxidative stress, apoptosis