Basic Research - Neurourology & LUTS/BPH & Others(구연) (E-036) Efficacy of combination therapy with pentosane polysulfate sodium and adipose tissue-derived stem cells for the management of interstitial cystitis in a rat model 경북대학교병원 비뇨의학과¹ 칠곡경북대학교병원 비뇨의학과² 경북대학교 의과대학 비뇨기과학교실³ 민경찬¹, 박경민², 이승윤², 권태균²³, 김태환²³, 유은상¹³, 이준녕²³, 하윤석²³, 정재욱², 김범수¹³ Introduction : Interstitial cystitis (IC) is one of the most intractable urologic disease. Although stem cell-based research has been showed promising results, possible recurrence after cell administration or insufficient long-term therapeutic effect might be expected. Therefore, we evaluated the synergistic effects of pentosane polysulfate sodium (PPS) and mesenchymal stem cells (MSCs) in an IC rat model. Materials and methods : After generation of the IC rat model, the rats were divided into 4 groups according to the treatment they received: phosphate-buffered saline (PBS) injection into bladder submucosa (control group), daily oral PPS feeding (PPS group), MSC injection into bladder submucosa (MSC group), or MSC injection into bladder submucosa with daily oral PPS feeding (MSC with PPS group). At 3, 7, 14 days after treatment, conscious cystometry and pain scale measurement were performed and their bladders were obtained for histological and proinflammatory-related gene expression analysis. Results : On cystometric analysis, all treatment groups showed significantly increased intercontraction intervals and lower pain scores compared to those of the control group. Histological analysis revealed regenerated urothelium, less fibrosis, and decreased mast cell infiltration in the treatment groups compared to the control group. Significantly lower expression of TNF-α, IFN-γ, MCP, IL-6, TLR2, and TLR11 was observed in the PPS with MSC group compared to the other groups. Conclusions : Combination therapy with PPS and MSCs showed histological and functional effects in an IC rat model, including synergistic effects leading to increased intercontraction interval and decreased inflammatory reactions. keywords : pentosan polysulfate sodium, adipose-tissue derived stem cell, interstitial cystitis