Basic Research - Neurourology & LUTS/BPH & Others(구연) (E-042) Extracorporeal shockwave therapy reduced NLRP3 inflammasome via inhibiting BAX/BAK pathway in prostatitis. 가톨릭대학교 서울성모병원 성재우, 권혁재, 신동호,허경재, 문형우, 박용현, 조혁진, 하유신, 홍성후, 이지열, 김세웅, 배웅진 Aim: The aim of this study was to evaluate the anti-inflammatory and antioxidative effects of extracorporeal shockwave therapy (ESWT) on prostatitis and explore the mechanism. Material & method: RWPE-1 cells randomly divided into 3 groups: 1 RWPE-1 group (normal control), 2 LPS group (lipopolysaccharide) and 3 ESWT group (LPS treated by ESWT). After ESWT administered, cells and supernatant were collected for ELISA and western blot. In vivo, Sprague-Dawley rats (n=36) were randomly divided into 3 groups: 1 normal group, 2 prostatitis group, and 3 ESWT group (12 for each). Prostatitis were induced by 17 beta-estradiol and dihydrotestosterone. 4 weeks after ESWT, pain index was assessed for all groups and prostates were collected for immunohistochemistry, western blot and ELISA. Results: ESWT ameliorated discomfort caused by prostatitis in rats. Compared to normal rats, overexpressed NLRP3 inflammasomes triggered apoptosis in inflammatory and oxidative microenvironment (P<0.01). Inflammation was improved by ESWT in prostatitis rats (P<0.05). TLR4-NFκB pathway was overactive with prostatitis, compared to normal and ESWT group (P<0.01). ESWT promoted prostate tissue recovery by stimulating VEGF overexpression (P<0.01). ESWT reduced NLRP3 inflammasomes in vivo via inhibiting BAX/BAK pathway. Conclusion: ESWT reduced NLPR3 inflammasome via inhibiting BAX/BAK pathway, which improved inflammation and ameliorated oxidative stress injury in prostatitis rats. keywords : ESWT, NLRP3 inflammasome, Chronic prostatitis