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이분척추 소아환자에서 분변미생물의 차이: 전향적 환자 대조군 연구 |
| 연세대학교 의과대학 비뇨의학교실, ¹소아과학교실, ²일산병원 비뇨의학과 |
| 이용승, 박소원¹, 강숭구², 김상운, 고홍¹, 한상원 |
Purpose: The composition of fecal microbiota is changed after spinal cord injury. The change of microbiota has been known to affect and be affected by host immunity and metabolism in patients with spinal cord injury. Nevertheless, the fecal microbiota composition in patients with spina bifida, the congenital disease of spinal cord has never been reported. Considering the difference of metabolism, infection, mood, and life style in children with spinal cord injury, we hypothesized the significant change in the composition of their gut microbiota and performed comparative analysis of them and healthy controls.
Methods: We performed prospective case-control study on 30 children (3-12 years old) with spinal bifida and 10 healthy controls (Clinical trial.gov, NCT 04186130). The composition of fecal microbiota was analyzed in genus level with 16S rRNA sequencing.
Results: Faecalibacterim and Bacteroides were significantly more abundant in control group. On linear discriminant analysis, Bifidobacterium and Lactobacillus were related with spinal bifida group. Butyrate producing bacterias such as Faecalibacterium, Bacteroides, Lachnospiraceae, and Roseburia were related to normal control.
Conclusion: Our results demonstrate for the first time that butyrate-producing bacterias were reduced in children with spinal bifida. It is consistent with previous results in patients with spinal cord injury. |
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keywords : spina bifida, neurogenic bladder, microbiota |
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