Basic Research - Neurourology & LUTS/BPH & Others(구연) (E-028) Bioinformatics Approach for Identifying Novel Biomarkers and Their Signaling Pathways Involved in Interstitial Cystitis/Bladder Pain Syndrome with Hunner Lesions Urology, Konkuk University Medical Center, Urology, Konkuk University Medical Center, Urology, Konkuk University Medical Center, Urology, Konkuk University Medical Center, Urology, Konkuk University Medical Center, Urology, Konkuk University Medical Center, Urology, Konkuk University Medical Center Aram Kim, Hyeong Gon Kim, Hyun Woo Kim, Jae-Hak Ahn, Woo-Suk Choi, HyungGuen Park, Sung Hyun Paick Background: The complexity of interstitial cystitis/bladder pain syndrome (IC/BPS) has led to considerable uncertainty in terms of diagnosis and prevalence of the condition. / Objectives: To identify the IC/BPS-associated genes through an integrated analysis of Gene Expression Omnibus (GEO) datasets and confirm experimentally to predict the pathologic diagnosis of IC/BPS. / Materials and Methods: Data mining analysis of GEO datasets (GSE621, GSE11783, GSE28242, and GSE57560) revealed a total of 53 (51 up-regulated and 2 down-regulated) common differentially expressed genes (DEGs) in IC/BPS. A protein-protein interaction (PPI) network was then constructed with the 53 common DEGs using Cytoscape v3.7.2, and subsequently, 6 hub genes (CD5, CD38, ITGAL, IL7R, KLRB1, and IL7R) were identified using cytoHubba v0.1 that were upregulated in IC/BPS. Results: Enrichment analysis of common DEGs revealed that hematopoietic cell lineage, immune system, and TCR signaling in naïve CD4+ T cell signaling pathways were prominently involved with the common 51 up-regulated DEGs. The 2 common down-regulated DEGs may enrich linoleic acid metabolism and synthesis of epoxy (EET) and dihydroxyeicosatrienoic acid (DHET) signaling pathways in IC/BPS. Moreover, our RT-PCR data confirmed that the expression of the five hub genes (CD38, ITGAL, IL7R, KLRB1, and IL7R) was significantly augmented in IC/BPS patients’ samples when compared with their normal counterparts. / Results and Conclusion: We systematically predicted the significant biomarkers and possible signaling pathways involved in IC/BPS, confirming the differential expression of the hub genes in tissue samples from patients with IC/BPS. Thus, the hub genes might be used as potential diagnostic biomarkers of IC/BPS.