Basic Research - Cancer(구연) (E-007) Identification of LIMK2 as a novel prognostic marker and therapeutic target of clear cell renal cell carcinoma Urology, Samsung Medical Center, Life Systems, Sookmyung Women's Universtiy, Urology, Samsung Medical Center, Urology, Samsung Medical Center, Urology, Samsung Medical Center, Urology, Samsung Medical Center, Urology, Samsung Medical Center, Urology, Samsung Medical Center, Life Systems, Sookmyung Women's Universtiy, Urology, Samsung Medical Center Jungyu Kim, Je Yeong Ko, Hyun Hwan Sung, Hwang Gyun Jeon, Byong Chang Jeong, Seong Il Seo, Seong Soo Jeon, Hyun Moo Lee, Jong Hoon Park, Minyong Kang Background: Clear cell renal cell carcinoma (ccRCC) is the most common histologic subtype of renal cell carcinoma (RCC) with high incidence of local recurrence and distant metastasis. LIM-domain kinase 2 (LIMK2) has been shown to be involved in cell proliferation, migration, and invasion in different types of cancer; however, its roles in ccRCC remain unknown. / Objectives: In this study, we aimed to identify the role of LIMK2, and to confirm whether LIMK2 expression is correlated with the prognosis of patients with ccRCC. / Materials and Methods: We prospectively collected ccRCC tissues and matched normal tissues from 30 patients who underwent radical nephrectomy at our institution. The expressions of LIMK2 in these samples and ccRCC cell lines (ACHN and 786-O) were examined by real-time PCR and western blot assay. Furthermore, we analyzed KIRC-TCGA dataset from the public database to validate LIMK2 expression, and investigated its relationship with survival outcome of ccRCC patients. Finally, the biological functions of LIMK2 in ccRCC were demonstrated by CCK-8, colony formation, and transwell migration assay. / Results and Conclusion: Results: Our results showed that LIMK2 was significantly upregulated in most ccRCC tissues among 30 patients and cell lines. In addition, TCGA data showed that ccRCC patients with high expression of LIMK2 tended to have a higher nuclear grade and worse overall survival than those with lower expression (Figure 1). Moreover, we found that LIMK2 knockdown by using siRNA significantly inhibited cell proliferation, colony formation, and migration of ccRCC cell line (786-O) (Figure 2). Conclusions: In conclusion, our data showed that LIMK2 was upregulated in ccRCC, and higher expression of LIMK2 was correlated with higher tumor grade and poor survival outcome in patients with ccRCC. This study might provide LIMK2 as a novel prognostic biomarker and potential therapeutic target for ccRCC.   keywords : LIMK2, prognostic marker, renal cell carcinoma